Cheese culture media

Introduction

CSK food enrichment has a long tradition in the dairy industry as a producer of dairy ingredients. The expertise in bulk starter production for cheese is used for the development of a broad portfolio of culture media, which are optimally adjusted to the existing Ceska®-star bulk starter cultures.

Characteristics and properties

Culture media are a complex blend of skimmed milk powder, whey proteins, phosphate salts, yeast extracts and other specific growing agents. Culture media are used to improve bulk starter production of cheese as an alternative for skimmed milk to ensure a robust system with a consistent acid production, predictable activity and a high protection against phage infection.

Benefits of Ceska®-media

Improved cultures yield
The growth of the culture is maximised by optimal nutrition and growth conditions, due to:

  • fast adaptation of the culture after inoculation;
  • presence of specific minerals and compounds, which stimulate bacterial growth;
  • a buffered acidity system, which maximises the bacteria cell counts;
  • culture media are free of any inhibitors like e.g. antibiotics.

Protection against bacteriophage infection
Ceska®-media contains special phosphate salts, which reduce the amount of available Ca2+ ions. In this way bulk starter becomes less susceptible to bacteriophage infections.
Besides, the presence of phosphate salts buffers the acidity, which results in an increase in cell counts and thus a more robust system in case of high bacteriophage infection.

Figure 1. Final pH of bulkstarter at different initial phage infection loads

Figure 2. Activity of bulkstarter produced with different initial phage infection loads

 

Fig. 1. illustrates the effect of initial phage infection load of the bulk starter on the final pH after incubation: final pH of the bulk starter is not influenced by a high phage infection load when produced on medium, whereas the final pH stops at a higher level when milk is used as growing medium.
 

When the effect of initial phage infection load on final bulk starter activity is analysed (see fig. 2.), it shows that the activity of bulk starter is more stable at increased initial phage infection load when medium is used instead of milk before a decrease in activity is observed.

 

Consistent acidification performance
Due to optimal nutrition and growth conditions a robust system is ensured. This results in a consistent acidification performance, a predictable activity and thus an improved final product consistency.

Availability
With Ceska® -media all milk is available for production, which makes planning of the amount of liquid skimmed milk for bulk starter production not necessary.

Cost effective
An increased culture yield can also be used to reduce the percentage of culture addition up to 20-30%. This results in an energy and cost reduction on the one hand and a capacity increase on the other hand.

Quality of bulk starter
As the bulk starter reaches its optimum at a higher pH with Ceska®-media than with skimmed milk, the vitality and activity of the bulk starter is higher for longer periods.

Media types

Ceska®-media are available in a dust free and a dust reduced version. The dust reduced version is specific developed for addition via triblender, whereas the dust free version is agglomerated and specific developed for addition via manhole. The agglomerated version also has a higher solubility.

For every cheese type a specific medium is developed, which is optimal adjusted to the related Ceska®-star cultures. These media vary in buffering level, ingredient composition and dissolving concentration.

Microbiological composition

Total plate count : <20.000 cfu/g
Aerobic sporeforming  bacteria : < 100 cfu/g
Yeasts and moulds : < 10 cfu/g
Enterobacteriaceae : < 10 cfu/g
Salmonella : absent  in 25 g
Listeria monocytogenes : absent in 1 g
Coagulase-positive staphylococci : absent in 1 g

Quality Control

Ceska®-media are delivered with a high and constant quality thanks to quality control procedures (ISO 9001). Ceska®-media is carefully checked for its physical-chemical and microbiological properties. Strict control of the production process and standardisation procedures result in an end product that meets the HACCP requirements and all the current high quality demands. The procedures used by CSK are conform international guidelines and recommendations.

Packaging

The culture media within the Ceska®-media range are delivered as a powder in 25 kg or 12,5 kg bags of paper with polyethylene inlay or in big bags with variable content.

Storage and shelf life

The quality of Ceska®-media is guaranteed for 9 months after production, provided it is stored in the closed original packaging under cool and dry conditions at ambient temperatures.

Consultancy

The technical sales staff of CSK can help you in the application of cultures, culture media and facilitating your bulk starter production room. Besides that, we can help you implementing the NIZO procedure and optimising your process, especially the dry solids content in the butter.

This publication is solely intended for information purposes. CSK food enrichment cannot be held liable for the contents. This specifically applies in cases where its products are used in violation with prevailing legislation and rules and/or licenses.